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New Animal Model, Nanotechnologies and Neuroimaging to Study Interactions and Homing of Human Neural Stem Cells in Stroke
Apply Two Photon Microscopy to visualize morphological and functional aspects of brain physiology in cerebral infarction.
Two Photon Microscopy is an innovative technique to visualize in vivo biological tissues. It offers the chance of visualizing both morphological and functional aspects of brain physiology, in healthy as well as in pathological conditions, and combined with genetic techniques to target populations of neurons with fluorescent proteins, it allows to follow rearrangements of neural processes in the cortex of live animals.
In this project Two Photon Microscopy will be applied to visualize the temporal evolution of the neural network in an animal model of cerebral infarction. Main aim of researchers is to study the details of real dynamics of the homing process of transplanted stem cells that it has been shown may induce a functional recovery of the ischemic brain, migrating from the site of injection toward the lesion.
In vivo imaging of the inoculated stem cells, previously labeled with a fluorescent marker, in an animal model of ischemic disease will be performed, too. Thanks to previous technique it will be possible to follow the real dynamics of infiltrating cells through the brain parenchyma towards the site of injury, comparing these results with the behavior of these cells in an healthy environment.
Multidisciplinary background required to Two Photon Imaging technique is a key element to investigate the fundamental mechanisms of recovery after a stroke towards the development of novel therapeutic strategies.
Only publications with LENS-affiliated authors are listed and for now there is no one.
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Elenco Siti Tematici