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NEWS

Gil Bub

University of Oxford

Wednesday, July 6th, 2016 at 11:00:00 AM

Aula Querzoli - LENS

Published on-line at 04:03:35 PM on Tuesday, July 5th, 2016

New approaches for high speed imaging and control of cardiac dynamics

Imaging living heart tissue is challenging due to the wide range of space and time scales that are needed to resolve its structure and dynamics.

Imaging living heart tissue is challenging due to the wide range of space and time scales that are needed to resolve its structure and dynamics. Three emerging imaging modalities are discussed:Remote focusing microscopy, a multi-photon imaging modality that can rapidly scan a focal spot within a tissue volume, is used to measure sarcomere length (SL) from intact, Langendorff perfused hearts. The microscope's ability to image oblique planes is used to measure cell orientation, allowing SL to be unambiguously determined at high speed in intact tissue. Temporal pixel multiplexing (TPM), a new imaging modality that embeds temporal information in high-resolution images, is used to measure dynamics from voltage sensitive dye loaded cardiac preparations. Here, TPM resolved action potential upstroke velocities that are close to what is predicted in photon scattering simulations while maintaining high spatial resolution. Finally, a new dyeless imaging approach is demonstrated in conjuction with optogenetic control to visualize waves in cardiac monolayers. These three imaging spatiotemporal events in living tissue.

For further informations, please contact sacconi@lens.unifi.it.